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KMID : 0613820200300020122
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Journal of Life Science
2020 Volume.30 No. 2 p.122 ~ p.128
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Genetic Variations of Candida glabrata Clinical Isolates from Korea using Multi-locus Sequence Typing
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Kang Min-Ji
Lee Kyung-Eun
Jin Hyun-Woo
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Abstract
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Although Candida albicans is the major fungal pathogen of candidemia, severe infections by non-albicans Candida (NAC) spp. have been increasing in recent years. Among NAC spp., C. glabrata has emerged as the second most common pathogen. However, few studies have been conducted to investigate its structure, epidemiology, and basic biology. In the present study, multi-locus sequence typing (MLST) was performed with a total of 102 C. glabrata clinical isolates that were isolated from various types of clinical specimen. For MLST, six housekeeping genes?FKS, LEU2, NMT1, TRP1, UGP1, and URA3?were amplified and sequenced. The results were analyzed using the C. glabrata database. Out of a total of 3,345 base-pair DNA sequences, 49 variable nucleotide sites were found, and the results showed that 12 different sequence types (ST) were identified from the 102 clinical isolates. The data also demonstrated that the undetermined ST1 was the most predominant ST in Korea. Further, seven undetermined STs (UST) containing UST2-8 were classified at specific loci. The data from this study may provide a fundamental database for further studies on C. glabrata, including its epidemiology and evolution. The data may also contribute to the development of novel antifungal agents and diagnostic tests.
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KEYWORD
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Candida glabrata, non-albicans Candida, candidiasis, genetic variations, multi-locus sequence typing
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